Estrogen Receptor alpha Antikörper

Produktnummer ABIN6937185

Der Maus Monoklonal anti-Estrogen Receptor alpha Antikörper wird verwendet zum Nachweis von Estrogen Receptor alpha in Proben von Human. Er wurde validiert für WB und IHC.

Kurzübersicht für Estrogen Receptor alpha Antikörper (ABIN6937185)

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Estrogen Receptor alpha Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Klon: ESR1-1904

Produktdetails anti-Estrogen Receptor alpha Antikörper

Verwendungszweck: Estrogen Receptor, alpha (Marker of Estrogen Dependence) Antibody

Spezifität: This monoclonal antibody is specific to estrogen receptor alpha (ER alpha) and shows minimal cross-reaction with other members of the family. ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy. It strongly stains nuclei of epithelial cells in breast carcinomas.

Kreuzreaktivität (Details): Human.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human Estrogen Receptor alpha protein

Isotyp: IgG2a, kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: MCF-7 cells. Breast or Endometrial Carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Store at 2 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Estrogen Receptor alpha

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Andere Bezeichnung: ESR1

Hintergrund:

Estrogen Receptor alpha delta 4*5,6,7*/654 isoform, Estrogen Receptor alpha delta 4 +49 isoform, Nuclear receptor subfamily 3 group A member 1

MCF-7 cells. Breast or Endometrial Carcinoma.

Molekulargewicht: ~67kDa

Gen-ID: 2099, 208124

UniProt: P03372

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly