Emerin Antikörper (AA 56-167)

Produktnummer ABIN6937145

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Emerin in WB, IF und IHC (f). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Emerin Antikörper (AA 56-167) (ABIN6937145)

Target: Emerin (EMD)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Emerin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: EMD-2167

Produktdetails anti-Emerin Antikörper

Bindungsspezifität: AA 56-167

Spezifität: Emerin is a member of the nuclear lamina associated protein family. It is ubiquitously expressed and localized to the nuclear membrane in normal cells. Mutations of the gene that encodes emerin result in the X-linked recessive disease Emery-Dreifuss muscular dystrophy (EDMD), which is characterized by slowly progressing contractures, skeletal muscle wasting and cardiomyopathy. Reportedly, lack of Emerin expression is one cause of EDMD. Emerin is involved in the association of the nuclear membrane with the lamina, and is localized specifically to desmosomes and fasciae adherents in the heart. Identification of nuclear membrane irregularities with anti-emerin antibody has been reported useful in diagnosing papillary thyroid carcinoma.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human Emerin protein fragment (around aa 56-167) (exact sequence is proprietary)

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Western Blot (1-2 μg/mL), Immunofluorescence (1-3 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: K-562, HeLa or Jurkat cells. Kidney or Skin.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu Emerin

Target: Emerin (EMD)

Andere Bezeichnung: EMD

Hintergrund: EMD, Emerin, Emery Dreifuss muscular dystrophy (EDMD), STA,Emerin (Papillary Thyroid Carcinoma and EDMD Marker)
Cellular localisation: Nuclear Membrane

Molekulargewicht: 37kDa

Gen-ID: 2010, 522823

UniProt: P50402