Desmoglein 1 Antikörper (Intracellular)

Produktnummer ABIN6937108

Dieses Anti-Desmoglein 1-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Desmoglein 1 in IHC (f). Geeignet für Human.

Kurzübersicht für Desmoglein 1 Antikörper (Intracellular) (ABIN6937108)

Target: Desmoglein 1 (DSG1)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Desmoglein 1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: DSG1-1733

Produktdetails anti-Desmoglein 1 Antikörper

Bindungsspezifität: Intracellular

Spezifität: Recognizes a protein of ~150 kDa, identified as Desmoglein-1 (DSG1). Desmoglein-1 is a member of the desmosomal cadherin family.Desmosomes are intercellular adhering junctions that represent cell surface attachment sites for intermediate filament. Desmocollins and desmogleins are the main desmosomal transmembrane proteins. Desmogleins consist of Dsg1, Dsg2, Dsg3, and Dsg4 isoforms.Within the desmosome, the extracellular domain of desmoglein is essential for calcium dependent heterophilic binding to the desmocollins, whereas the intracellular domain is essential for binding to the desmosomal plaque protein, plakoglobin. Desmoglein 1 is synthesized exclusively in the suprabasal layers. Intact and functionally active desmoglein 1 is essential to epidermal integrity.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human desmoglein-1 protein fragment corresponding to intracellular domain

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris buffer with 1 mM EDTA, pH 8.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive_Control: A431 or A-375 cells. Skin.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu Desmoglein 1

Target: Desmoglein 1 (DSG1)

Andere Bezeichnung: DSG1

Hintergrund: Cadherin family member 4, CDHF4, Desmoglein-1, Desmosomal glycoprotein 1, DG1, DSG1, EPKHE, EPKHIA, Pemphigus foliaceus antigen, PPKS1, SPPK1,Desmoglein-1 (DSG1)
Cellular localisation: Cell surface

Molekulargewicht: 150kDa (precursor), 160kDa (mature)

Gen-ID: 1828, 2633

UniProt: Q02413

Pathways: Cell-Cell Junction Organization