Desmin Antikörper

Produktnummer ABIN6937087

Der Maus Monoklonal anti-Desmin Antikörper (Klon DES-1711) (ABIN6937087) detektiert spezifisch Desmin in WB, IHC, IF und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Ratte, Maus, Huhn und Hamster.

Kurzübersicht für Desmin Antikörper (ABIN6937087)

Target: Desmin (DES)

Reaktivität: Human, Ratte, Maus, Huhn, Hamster

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Desmin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: DES-1711

Produktdetails anti-Desmin Antikörper

Verwendungszweck: Desmin (Muscle Cell Marker) Antibody

Spezifität: Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. IFs are constructed from two-chain -helical coiled-coil molecules arranged on an imperfect helical lattice, and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. Vimentin is an IF general marker of cells originating in the mesenchyme. Vimentin and Desmin, a related class III IF, are both expressed during skeletal muscle development. Desmin, a 469 amino acid protein found near the Z line in sarcomeres, is expressed more frequently in adult differentiated state tissues. Anti-desmin detects cells of normal smooth, skeletal, and cardiac muscles.Antibody reacts with leiomyomas, leiomyosarcoma, rhabdomyomas, rhabdomyosarcoma, and perivascular cells of glomus tumors of the skin.

Kreuzreaktivität (Details): Human. Mouse. Rat. Hamster. Chicken.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human desmin protein

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunofluorescence (1-2 μg/mL), Flow Cytometry (1-2 μg/million cells), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: SJRH30 cells. Uterus or Leiomyosarcoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Desmin

Target: Desmin (DES)

Andere Bezeichnung: DES

Hintergrund:

CMD1I, CSM1, CSM2, DES, Intermediate filament protein

SJRH30 cells. Uterus or Leiomyosarcoma.

Molekulargewicht: 52kDa

Gen-ID: 1674, 594952

UniProt: P17661