Rekombinanter Cytokeratin, Multi (Epithelial Marker) Antikörper

Produktnummer ABIN6937080

Produktdetails

Target: Cytokeratin, Multi (Epithelial Marker)

Antikörpertyp: Recombinant Antibody

Reaktivität: Rind (Kuh), Frosch, Ziege, Meerschweinchen, Human, Affe, Maus, Schwein, Ratte

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Immunohistochemistry (IHC), Staining Methods (StM), Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)

Spezifität: Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, including 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 52 kDa (CK8), 56.5 kDa (CK10), 53 kDa (CK13) and 45 kDa (CK18). This is a broad-spectrum antibody, which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Aufreinigung: Purified by Protein A/G

Immunogen: Keratin-enriched preparation from cultured human epithelial cells

Klon: KRT-1877R

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Positive Control: A431 cells, Skin, Colon carcinoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1.0 μg/mL), Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 10 mM PBS without BSA and without Azide.

Konservierungsmittel: Azide free

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months.

Haltbarkeit: 24 months

Antigendetails

Target: Cytokeratin, Multi (Epithelial Marker)

Gen-ID: 3851, 3852, 3853

UniProt: P02538, P04259, P13647, P19013, P48668