Rekombinanter Cytokeratin (KRT) Antikörper

Produktnummer ABIN6937080

Der Kaninchen Monoklonal anti-Cytokeratin Antikörper wird verwendet zum Nachweis von Cytokeratin in Proben von Human, Rind (Kuh), Frosch, Ziege, Meerschweinchen, Affe, Maus, Schwein und Ratte. Er wurde validiert für WB, IHC, FACS und IF.

Kurzübersicht für Rekombinanter Cytokeratin (KRT) Antikörper (ABIN6937080)

Target: Cytokeratin (KRT)

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Rind (Kuh), Frosch, Ziege, Meerschweinchen, Affe, Maus, Schwein, Ratte

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: KRT-1877R

Produktdetails

Verwendungszweck: Recombinant Cytokeratin, Multi (Epithelial Marker) Antibody

Spezifität: Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, including 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 52 kDa (CK8), 56.5 kDa (CK10), 53 kDa (CK13) and 45 kDa (CK18). This is a broad-spectrum antibody, which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Keratin-enriched preparation from cultured human epithelial cells

Isotyp: IgG, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: A431 cells, Skin, Colon carcinoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1.0 μg/mL), Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Antigendetails

Target: Cytokeratin (KRT)

Andere Bezeichnung: Cytokeratin

Hintergrund: A431 cells, Colon carcinoma., Skin

Gen-ID: 3851, 3852, 3853

UniProt: P02538, P04259, P13647, P19013, P48668