Keratin 5,6,18 Antikörper

Produktnummer ABIN6937074

Der Maus Monoklonal Anti--Antikörper wurde für IHC und StM validiert. Er ist geeignet, in Proben von Human, Ratte und Rind (Kuh) zu detektieren.

Kurzübersicht für Keratin 5,6,18 Antikörper (ABIN6937074)

Target: Keratin 5,6,18

Reaktivität: Human, Ratte, Rind (Kuh)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Immunohistochemistry (IHC), Staining Methods (StM)

Klon: LP34

Produktdetails

Spezifität: It recognizes polypeptides of 58 kDa, 56 kDa, and 45 kDa, identified as cytokeratin 5, 6, and 18 respectively. It shows no reaction with keratin 1, 8, or 19. This MAb shows a broad pattern of reactivity with epithelial tissues, from simple glandular epithelia to stratified squamous epithelia. Epithelial cells are labeled whether they are ectodermal, mesodermal, or endodermal in origin. This MAb is useful in detecting cells of epithelial origin in mixed tumors, in effusions, in bone marrow samples, or in tissue culture cells.

Aufreinigung: Purified by Protein A/G

Immunogen: Detergent-insoluble fraction of psoriatic human epidermis

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: HeLa, HepG2 or PC12 cells. Tonsil or Skin.

Known Application: Immunohistochemistry (Formalin-fixed) (0.1-0.2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 10 mM PBS without BSA and without Azide.

Konservierungsmittel: Azide free

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months.

Haltbarkeit: 24 months

Antigendetails

Target: Keratin 5,6,18

Andere Bezeichnung: Cytokeratin 5/6/18

Molekulargewicht: 45-58kDa