CNN1 Antikörper

Produktnummer ABIN6937010

Produktdetails anti-CNN1 Antikörper

Target: CNN1 (Calponin 1 (CNN1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CNN1 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Spezifität: Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist, a 34 kDa form and a 29 kDa form. Expression of the 29 kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, this antibody reacts with only the 34 kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.

Kreuzreaktivität (Details): Human. Rat.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human CNN1 protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 1 mM EDTA, pH 7.5-8.5, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive_Control: Myoepithelial cells in breast ducts.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu CNN1

Target: CNN1 (Calponin 1 (CNN1))

Andere Bezeichnung: CNN1 (CNN1 Produkte)

Synonyme: CN antikoerper, CnnI antikoerper, SMCC antikoerper, Sm-Calp antikoerper, XclpH3 antikoerper, clpH3 antikoerper, cnn2 antikoerper, calponin 1 antikoerper, calponin 1, basic, smooth muscle L homeolog antikoerper, cnn1 antikoerper, Cnn1 antikoerper, CNN1 antikoerper, cnn1.L antikoerper

Hintergrund: Calponin 1 basic smooth muscle, Calponin H1 smooth muscle, Calponin-1, CNN1, Cnn1, Sm Calp, SMCC,Calponin-1 (Smooth Muscle Marker)
Cellular localisation: Cytoplasmic

Molekulargewicht: 34kDa

Gen-ID: 1264, 465929

UniProt: P51911