Rekombinanter CNN1 Antikörper

Produktnummer ABIN6937009

Dieses Anti-CNN1-Antikörper ist ein Kaninchen Monoklonal-Antikörper zur Detektion von CNN1 in FACS, IF und IHC (f). Geeignet für Human.

Kurzübersicht für Rekombinanter CNN1 Antikörper (ABIN6937009)

Target: CNN1 (Calponin 1 (CNN1))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser CNN1 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: CNN1-1408R

Produktdetails anti-CNN1 Antikörper

Spezifität: Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist, a 34 kDa form and a 29 kDa form. Expression of the 29 kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, this MAb reacts with only the 34 kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.

Kreuzreaktivität (Details): Human. Rat.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human full-length CNN1 protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive_Control: K562 cells. Myoepithelial cells in breast ducts.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu CNN1

Target: CNN1 (Calponin 1 (CNN1))

Andere Bezeichnung: CNN1

Hintergrund: Calponin 1 basic smooth muscle, Calponin H1 smooth muscle, Calponin-1, CNN1, Cnn1, Sm Calp, SMCC,Calponin-1 (Smooth Muscle Marker)
Cellular localisation: Cytoplasmic

Molekulargewicht: 34kDa

Gen-ID: 1264, 465929

UniProt: P51911