CLTA/CLTB Antikörper (N-Term)

Produktnummer ABIN6937002

Der Maus Monoklonal Anti-CLTA/CLTB-Antikörper wurde für WB, FACS, IF und IHC (f) validiert. Er ist geeignet, CLTA/CLTB in Proben von Human zu detektieren.

Kurzübersicht für CLTA/CLTB Antikörper (N-Term) (ABIN6937002)

Target: CLTA/CLTB (Clathrin, Light Chain A/B (CLTA/CLTB))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CLTA/CLTB Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: SPM174

Produktdetails anti-CLTA/CLTB Antikörper

Bindungsspezifität: N-Term

Spezifität: Recognizes proteins of 31-44 kDa, which are identified as Clathrin Light Chains (both A B). Clathrin is composed of three heavy chains and three light chains, which associate non-covalently to form a triskelion structure. Clathrin light chain regulates the self-assembly of triskelions onto intracellular membranes. Clathrin light chain subunits (LCA and LCB) contribute to regulation of coated vesicle formation to sort proteins during receptor-mediated endocytosis and organelle biogenesis. LCA and LCB are encoded by two discrete genes. They share only 60 % homology, and have certain features in common. Both LCA and LCB undergo alternative mRNA splicing, which results in the generation of tissue-specific isoforms.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Purified recombinant N-terminal fragment of human Clathrin Light Chain

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HeLa cells. Placenta or Prostate Carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu CLTA/CLTB

Target: CLTA/CLTB (Clathrin, Light Chain A/B (CLTA/CLTB))

Andere Bezeichnung: CLTA and CLTB

Hintergrund: Clathrin light chain A, Clathrin light chain B, Clathrin light chain LCA, Clathrin light chain LCB, Clathrin light polypeptide A, Clathrin light polypeptide B, Clathrin, light polypeptide (Lca), Clathrin, light polypeptide (Lcb), CLTA, CLTB,Clathrin, Light Chain
Cellular localisation: Cytoplasmic

Molekulargewicht: 31-44kDa

Gen-ID: 1211, 1212, 484241

UniProt: P09496, P09497