CHD4 Antikörper (C-Term)

Produktnummer ABIN6936972

Der Maus Monoklonal Anti-CHD4-Antikörper wurde für WB, ELISA, IF, FACS, Coat und IHC (f) validiert. Er ist geeignet, CHD4 in Proben von Human zu detektieren.

Kurzübersicht für CHD4 Antikörper (C-Term) (ABIN6936972)

Target: CHD4 (Chromodomain Helicase DNA Binding Protein 4 (CHD4))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CHD4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: 3F2-4

Produktdetails anti-CHD4 Antikörper

Bindungsspezifität: C-Term

Spezifität: CHD4/Mi-2b is a component of the nucleosome remodeling and deacetylase (NuRD) complex which is a multi-subunit protein complex containing both histone deacetylase and nucleosome-dependent ATPase subunits. Current models predict that this complex function primarily in transcriptional repression. Accumulating evidence indicates that NuRD may regulate the transcription of specific genes by interacting with specific transcriptional factors. In addition, the NuRD complex may also participate in genome-wide transcriptional regulation through an association with histone tails.

Kreuzreaktivität (Details): Human. Mouse.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Synthetic peptide (N- and C- terminal peptides were used for immunisation: 1. ASGLGSPSPCSAGSEEEDM & 2. CSRLANRAPEPPPQQVAQQQ) - which peptide the antibody recognises has not been tested.

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: ELISA (For coating, order antibody without BSA), Western Blot (1-2 μg/mL)Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HeLa whole cell lysate or human placenta tissue lysate. Human breast carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu CHD4

Target: CHD4 (Chromodomain Helicase DNA Binding Protein 4 (CHD4))

Andere Bezeichnung: CHD4

Hintergrund: ATP dependent helicase CHD4, Chromodomain helicase DNA binding protein 4, Mi 2 autoantigen 218 kDa protein, Mi 2b, Mi2 beta,CHD4
Cellular localisation: Nucleus and cytoplasm.

Molekulargewicht: 238kDa

Gen-ID: 1108, 162233

UniProt: Q14839