CGA (HCG-Alpha) & CGB (HCG-Beta) Antikörper

Produktnummer ABIN6936962

Dieser Maus Monoklonal Antikörper detektiert spezifisch CGA (HCG-Alpha) & CGB (HCG-Beta) in IHC. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für CGA (HCG-Alpha) & CGB (HCG-Beta) Antikörper (ABIN6936962)

Target: CGA (HCG-Alpha) & CGB (HCG-Beta)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Immunohistochemistry (IHC)

Klon: HCGab-52

Produktdetails

Verwendungszweck: HCG Holo (Pregnancy & Choriocarcinoma Marker) Antibody

Spezifität: This MAb is a very special because it reacts ONLY with the intact-HCG and not with either free alpha- or free beta-chain of HCG. HCG is a glycoprotein and is composed of two non-identical, non-covalently linked polypeptide chains designated as the subunit is identical to that of thyroid stimulating hormone (TSH) follicle stimulating hormone (FSH), and luteinizing hormone (LH). HCG is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG MAb detects cells and tumors of trophoblastic origin such as choriocarcinoma. Large cell carcinoma and adenocarcinoma of the lung demonstrate anti-hCG positivity in 90 % and 60 % of cases respectively. 20 % of lung squamous cell carcinomas are positive. HCG expression by non-trophoblastic tumors may indicate aggressive behavior.

Kreuzreaktivität (Details): Human.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Purified hCG protein

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: JAR or TT cells. Placenta.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Antigendetails

Target: CGA (HCG-Alpha) & CGB (HCG-Beta)

Hintergrund:

CG-alpha, CGA, Chorionic Gonadotrophin Alpha, Follicle Stimulating Hormone Alpha, Follitropin Alpha, FSH-alpha, FSHA, GPH Alpha, GPHA1, LHA, LH-alpha, Luteinizing Hormone Alpha, Lutropin Alpha, Thyroid Stimulating Hormone Alpha, Thyrotropin Alpha, TSHA, CG-beta, CGB3, CGB5, CGB7, CGB8, Choriogonadotropin Subunit beta, hCGB

JAR or TT cells. Placenta.

Molekulargewicht: ~35kDa (intact hCG)

Gen-ID: 1081, 1082, 172944

UniProt: P01215, P01233