Elastase 3B Antikörper (AA 82-238)

Produktnummer ABIN6936945

Der Maus Monoklonal Anti-Elastase 3B-Antikörper wurde für WB, ELISA, Coat und IHC (f) validiert. Er ist geeignet, Elastase 3B in Proben von Human zu detektieren.

Kurzübersicht für Elastase 3B Antikörper (AA 82-238) (ABIN6936945)

Target: Elastase 3B (ELA3B) (Elastase 3B, Pancreatic (ELA3B))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Elastase 3B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: CELA3B-1257

Produktdetails anti-Elastase 3B Antikörper

Bindungsspezifität: AA 82-238

Spezifität: This MAb recognizes a protein of ~30 kDa, identified as CELA3B (Chymotrypsin like elastase family member 3B). Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3B has little elastolytic activity. Like most of the human elastases, elastase 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. Elastase 3B may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays.

Kreuzreaktivität (Details): Human. Mouse. Rat.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human CELA3B protein fragment (around aa 82-238) (exact sequence is proprietary)

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA)Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Panc-28 or PANC1 cells. Pancreas.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu Elastase 3B

Target: Elastase 3B (ELA3B) (Elastase 3B, Pancreatic (ELA3B))

Andere Bezeichnung: CELA3B

Hintergrund: Chymotrypsin like elastase family member 3B (CELA3B), ELA3B, Elastase IIIB, Protease E,CELA3B / ELA3B (Pancreatic Function Marker)
Cellular localisation: Cytoplasmic

Molekulargewicht: ~17kDa

Gen-ID: 23436, 728752

UniProt: P08861