CD68 Antikörper (AA 150-301)

Produktnummer ABIN6936784

Dieses Anti-CD68-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von CD68 in FACS, ELISA, IHC (f) und Coat. Geeignet für Human.

Kurzübersicht für CD68 Antikörper (AA 150-301) (ABIN6936784)

Target: CD68 (CD68 Molecule (CD68))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD68 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), ELISA, Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Coating (Coat)

Klon: LAMP4-1830

Produktdetails anti-CD68 Antikörper

Bindungsspezifität: AA 150-301

Spezifität: This antibody recognizes a glycoprotein of 110 kDa, which is identified as CD68. It is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. It reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells, which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Lymphomas are negative or show few granules.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment of humanCD68 protein (around aa 150-301) (exact sequence is proprietary)

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Flow Cytometry (1-2 μg/million cells),ELISA (For coating, order antibody without BSA),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: U87MG, Tonsil, Lymph Node or Spleen.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu CD68

Target: CD68 (CD68 Molecule (CD68))

Andere Bezeichnung: CD68

Hintergrund: GP110, LAMP4, Microsialin, Macrosialin, SCARD1, Scavenger Receptor Class D Member-1,CD68 (Macrophage Marker)
Cellular localisation: Cell Surface and Cytoplasmic

Molekulargewicht: 110kDa

Gen-ID: 968, 647419

UniProt: P34810