CD63 Antikörper

Produktnummer ABIN6936774

Der Maus Monoklonal Anti-CD63-Antikörper wurde für FACS, IF, ISt und IHC (f) validiert. Er ist geeignet, CD63 in Proben von Human zu detektieren.

Kurzübersicht für CD63 Antikörper (ABIN6936774)

Target: CD63

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD63 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunostaining (ISt), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: NKI-C3

Produktdetails anti-CD63 Antikörper

Spezifität: This MAb recognizes protein of 26 kDa-60 kDa, which is identified as CD63. Its epitope is different from that of MAb LAMP3/529. The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.

Kreuzreaktivität (Details): Human and mouse.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Smooth plasma membrane fraction of MeWo cells

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HL60, THP-1 or NIH/3T3 cells. Human melanoma or lymphoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu CD63

Target: CD63

Andere Bezeichnung: CD63

Hintergrund: Gp55, granulophysin, Lysosomal-associated membrane protein 3 (LAMP-3), Mast cell antigen AD1, melanoma 1 antigen, Melanoma-associated antigen MLA1, Melanoma-associated antigen ME491, MLA1, NGA, Ocular melanoma-associated antigen, OMA81H, PTLGP40, Tetraspanin-30, TSPAN30,CD63 (Late Endosomes Marker)
Cellular localisation: Cell Surface. Cytoplasm.

Molekulargewicht: 26kDa (core protein), 30-60kDa (glycosylated)

Gen-ID: 967, 445570

UniProt: P08962