CD34 Antikörper

Produktnummer ABIN6936667

Dieses Anti-CD34-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von CD34 in FACS, IF und IHC (f). Geeignet für Human.

Kurzübersicht für CD34 Antikörper (ABIN6936667)

Target: CD34

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD34 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: SPM123

Produktdetails anti-CD34 Antikörper

Spezifität: This MAb recognizes a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34. On the basis of differential sensitivity to degradation by specific enzymes, epitopes of monoclonal antibodies to CD34 are classified intothree main categories, class I, class II and class III. It is a class II antibody whose epitope is resistant to neuraminidase but sensitive to glycoprotease and chymopapain.CD34 expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, CD34 is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. Its expression is also found in vascular endothelium. Additionally, it appears that proliferating endothelial cells express this molecule more than the non-proliferating endothelial cells. Anti-CD34 labels 85% of angiosarcoma and Kaposi's sarcoma, but with a lower specificity.

Keine Kreuzreaktivität: Rind (Kuh), Hund, Ratte (Rattus), Schaf

Kreuzreaktivität (Details): Human. Cynomolgus Monkey. Rhesus Monkey. Does not react with Rat, Sheep, Cow or Dog.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Detergent solubilized vesicular suspension prepared from human term placenta

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: KG-1 cells. Human tonsil or angiosarcoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu CD34

Target: CD34

Andere Bezeichnung: CD34

Hintergrund: Hematopoietic Progenitor Cell Antigen, HPCA1, Mucosialin,CD34 (Hematopoietic Stem Cell & Endothelial Marker)
Cellular localisation: Cell surface

Molekulargewicht: 90-110kDa

Gen-ID: 947, 374990

UniProt: P28906