CD19 Antikörper (AA 96-281)

Produktnummer ABIN6936615

Der Maus Monoklonal Anti-CD19-Antikörper wurde für FACS, IHC, ELISA, Coat und IHC (f) validiert. Er ist geeignet, CD19 in Proben von Human zu detektieren.

Kurzübersicht für CD19 Antikörper (AA 96-281) (ABIN6936615)

Target: CD19 (CD19 Molecule (CD19))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD19 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: CD19-3117

Produktdetails anti-CD19 Antikörper

Bindungsspezifität: AA 96-281

Spezifität: CD19 is a transmembrane glycoprotein that contains two extracellular immunoglobulin-like domains. CD19 is present in both benign and malignant B-cells and is considered to be the most reliable surface marker of this lineage over a wide range of maturational stages. In normal lymphoid tissue, CD19 is observed in germinal centers, in mantle zone cells, and in scattered cells of the inter-follicular areas. Anti-CD19 exhibits an overall immunoreactivity pattern similar to those of the antibodies against CD20 and CD22. However, in contrast to CD20, expression of CD19 is continuous throughout B-cell development and through terminal differentiation of B-cells into plasma cells. Anti-CD19 positivity is seen in the vast majority of B-cell neoplasms commonly at a lower intensity than normal B-cell counterparts. Plasma cell neoplasms are nearly always negative, as are T-cell neoplasms.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment of human CD19 protein (around aa96-281) (exact sequence is proprietary)

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: ELISA (For coating, order antibody without BSA), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Raji cells (FACS). Tonsil, Lymph Node or Spleen (IHC).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu CD19

Target: CD19 (CD19 Molecule (CD19))

Andere Bezeichnung: CD19

Hintergrund: B-lymphocyte antigen CD19, B-lymphocyte surface antigen B4, CVID3, Leu-12, T-cell surface antigen Leu-12,CD19 (B-Lymphocyte Marker)
Cellular localisation: Cell Surface

Molekulargewicht: 95kDa

Gen-ID: 930, 652262

UniProt: P15391

Pathways: Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung