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Biotin Antikörper

WB, FACS, IHC, IF, ISt, CM Wirt: Maus Monoclonal Hyb-8 unconjugated
Produktnummer ABIN6936505
  • Target Alle Biotin Produkte
    Biotin
    Reaktivität
    Bitte anfragen
    Wirt
    • 56
    • 34
    • 28
    Maus
    Klonalität
    • 75
    • 36
    Monoklonal
    Konjugat
    • 29
    • 11
    • 8
    • 8
    • 7
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser Biotin Antikörper ist unkonjugiert
    Applikation
    • 54
    • 49
    • 43
    • 35
    • 32
    • 19
    • 14
    • 12
    • 12
    • 12
    • 9
    • 9
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunostaining (ISt), Confocal Microscopy (CM)
    Spezifität
    It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
    Aufreinigung
    Purified by Protein A/G
    Immunogen
    Biotinylated sheep immunoglobulin
    Klon
    Hyb-8
    Isotyp
    IgG1 kappa
  • Applikationshinweise

    Positive Control: Biotinylated proteins in solution or on tissues.

    Known Application: Confocal Microscopy, Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), In situ Nucleic Acid Hybridization, Western Blot, Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT) (No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.)Optimal dilution for a specific application should be determined.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Buffer
    10 mM PBS without BSA and without Azide.
    Konservierungsmittel
    Azide free
    Lagerung
    4 °C,-80 °C
    Informationen zur Lagerung
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months.
    Haltbarkeit
    24 months
  • Target
    Biotin
    Andere Bezeichnung
    Biotin (Vitamin B7 or Vitamin H) (Biotin Produkte)
    Substanzklasse
    Chemical
    Molekulargewicht
    244Da
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