Biotin Antikörper
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- Target Alle Biotin Produkte
- Biotin
- Reaktivität
- Bitte anfragen
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser Biotin Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunostaining (ISt), Confocal Microscopy (CM)
- Spezifität
- It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
- Aufreinigung
- Purified by Protein A/G
- Immunogen
- Biotinylated sheep immunoglobulin
- Klon
- Hyb-8
- Isotyp
- IgG1 kappa
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- Applikationshinweise
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Positive Control: Biotinylated proteins in solution or on tissues.
Known Application: Confocal Microscopy, Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), In situ Nucleic Acid Hybridization, Western Blot, Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT) (No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.)Optimal dilution for a specific application should be determined.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Buffer
- 10 mM PBS without BSA and without Azide.
- Konservierungsmittel
- Azide free
- Lagerung
- 4 °C,-80 °C
- Informationen zur Lagerung
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months.
- Haltbarkeit
- 24 months
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- Target
- Biotin
- Andere Bezeichnung
- Biotin (Vitamin B7 or Vitamin H) (Biotin Produkte)
- Substanzklasse
- Chemical
- Molekulargewicht
- 244Da
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