alpha Fetoprotein Antikörper

Produktnummer ABIN6936369

Der Maus Monoklonal anti-alpha Fetoprotein Antikörper wird verwendet zum Nachweis von alpha Fetoprotein in Proben von Human, Schwein, Hund und Affe. Er wurde validiert für WB und IHC.

Kurzübersicht für alpha Fetoprotein Antikörper (ABIN6936369)

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Reaktivität: Human, Schwein, Hund, Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser alpha Fetoprotein Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Klon: C2

Produktdetails anti-alpha Fetoprotein Antikörper

Verwendungszweck: AFP (Alpha Fetoprotein) (Hepatocellular/Germ Cell Tumor Marker) Antibody

Spezifität: It recognizes an oncofetal glycoprotein with a single chain of 70 kDa, which is identified as alpha fetoprotein (AFP) (ISOBM TD-2 workshop). This MAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.

Keine Kreuzreaktivität: Rind (Kuh), Hund, Ratte (Rattus)

Kreuzreaktivität (Details): Human. Mouse. Does not react with Cow, Dog or Rat.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Alpha feto protein (AFP) Purified from serum of a hepatoma patient

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Hep-G2 cells. Fetal liver or hepatocellular carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu alpha Fetoprotein

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Andere Bezeichnung: AFP

Hintergrund:

Alpha fetoglobulin, FETA, HPAFP

Hep G2 cells. Fetal liver or hepatocellular carcinoma.

Molekulargewicht: 70kDa

Gen-ID: 174, 518808

UniProt: P02771

Pathways: C21-Steroid Hormone Metabolic Process