ACTN2 Antikörper (AA 557-692)

Produktnummer ABIN6936359

Produktdetails anti-ACTN2 Antikörper

Target: ACTN2 (Actinin, alpha 2 (ACTN2))

Bindungsspezifität: AA 557-692

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ACTN2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Spezifität: Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In non-muscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. Several transcript variants encoding different isoforms have been found for this gene.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: A recombinant fragment (aa557-692) of human ACTN2 protein (exact sequence is proprietary)

Klon: ACTN2-3292

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Skeletal or Cardiac Muscle.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu ACTN2

Target: ACTN2 (Actinin, alpha 2 (ACTN2))

Andere Bezeichnung: ACTN2 (ACTN2 Produkte)

Synonyme: MGC89234 antikoerper, ACTN2 antikoerper, 1110008F24Rik antikoerper, CMD1AA antikoerper, wu:fd44c03 antikoerper, zgc:123223 antikoerper, actinin alpha 2 antikoerper, actinin, alpha 2b antikoerper, actinin alpha 2 S homeolog antikoerper, actn2 antikoerper, ACTN2 antikoerper, Actn2 antikoerper, actn2b antikoerper, actn2.S antikoerper

Hintergrund: Actin binding protein, Alpha actinin skeletal muscle, isoform 2, CMD1AA, F-actin cross-linking protein,Sarcomeric Actinin Alpha 2 / ACTN2
Cellular localisation: Cytoplasmic

Molekulargewicht: 103kDa

Gen-ID: 88, 498178

UniProt: P35609

Pathways: Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Negative Regulation of Transporter Activity