ACP5 Antikörper

Produktnummer ABIN6936341

Dieses Maus Monoklonal-Antikörper erkennt spezifisch ACP5 in IHC (f). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für ACP5 Antikörper (ABIN6936341)

Target: ACP5 (Acid Phosphatase 5, Tartrate Resistant (ACP5))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ACP5 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: ACP5-1070

Produktdetails anti-ACP5 Antikörper

Spezifität: It recognizes a protein of 35kDa, which is identified as tartrate-resistant acid phosphatase (TRAcP). It exists as two isoforms (5a and 5b). This MAb reacts with both the isoforms. Serum TRAcP 5a is secreted by macrophages and dendritic cells and increased in many patients of rheumatoid arthritis.Serum TRAcP 5b is produced from osteoclasts and elevated during bone resorption. TRAcP is an iron containing glycoprotein, which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate. TRAcP is synthesized as a latent proenzyme and is activated by proteolytic cleavage and reduction. Normally, TRAcP is highly expressed by osteoclasts, activated macrophages, neurons and endometrium during pregnancy. Expression of TRAcP is increased in certain pathological conditions such as Leukemic Reticuloendotheliosis (Hairy Cell Leukemia), Gaucher's Disease, HIV-induced Encephalopathy, Osteoclastoma and in osteoporosis and metabolic bone diseases. Anti-TRAcP antibody labels the cells of Hairy Cell Leukemia (HCL) with a high degree of sensitivity and specificity. Other cells stained with this antibody are tissue macrophages and osteoclasts.

Kreuzreaktivität (Details): Human. Mouse. Rat.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human ACP5 protein

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HepG2, 293T, K562 or RPMI-8226 cells. Spleen from Hairy Cell Leukemia (HCL) patient.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu ACP5

Target: ACP5 (Acid Phosphatase 5, Tartrate Resistant (ACP5))

Andere Bezeichnung: ACP5

Hintergrund: Acid phosphatase 5, tartrate resistant, ACP5, serum band 5 tartrate-resistant acid phosphatase, SPENCDI, Tartrate-resistant acid ATPase, Tartrate-resistant acid phosphatase type 5, TR-AP, TRACP5, TRAcP, TRAP, TrATPase, Type 5 acid phosphatase,TRAcP (Tartrate-Resistant Acid Phosphatase) (Hairy Cell Leukemia Marker)
Cellular localisation: Cytoplasmic (Lysosomes)

Molekulargewicht: 35kDa

Gen-ID: 54, 1211

UniProt: P13686

Pathways: Transition Metal Ion Homeostasis