ABO Antikörper

Produktnummer ABIN6936339

Der Maus Monoklonal Anti-ABO-Antikörper wurde für AGG, H und IHC (f) validiert. Er ist geeignet, ABO in Proben von Human zu detektieren.

Kurzübersicht für ABO Antikörper (ABIN6936339)

Target: ABO (Histo-blood group A transferase (ABO))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ABO Antikörper ist unkonjugiert

Applikation: Agglutination (AGG), Haemagglutination (H), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: HEB-20

Produktdetails anti-ABO Antikörper

Spezifität: The antibody HEB-20 reacts with human blood group B. The specificity of the antibody HEB-20 was confirmed by comparison of specificity and reactivity to standard reagent using 5.000 samples of blood. The MAb HEB-20 shows specific staining of erythrocytes and vascular epithelium of blood group B controls and no staining in group A controls. This MAb is applicable for tissue staining in tumor patients with blood groups B and AB. Blood group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detected on erythrocytes and certain epithelial cells. The A, B and H antigens are reported to undergo modulation during malignant cellular transformation. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Mixture of erythrocytes of group B and glyco protein fraction isolated from saliva of secretors with blood group B

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Agglutination, Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: KG1 cells. Human colorectal carcinoma tissues.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu ABO

Target: ABO (Histo-blood group A transferase (ABO))

Andere Bezeichnung: ABO

Hintergrund: A transferase, ABO, B transferase, CD173, Fucosylglycoprotein 3-alpha-galactosyltransferase, Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase, Glycoprotein-fucosylgalactoside alpha-galactosyltransferase, Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase, Histo-blood group A transferase, Histo-blood group B transferase, NAGAT,Blood Group Antigen B (CD173)
Cellular localisation: Cell Surface

Molekulargewicht: Multiple

Gen-ID: 28, 654423

UniProt: P16442