Vimentin Antikörper (CF®488A)

Produktnummer ABIN6934573

Dieses Anti-Vimentin-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Vimentin in IF, FACS und T. Geeignet für Human, Schwein, Rind (Kuh), Huhn, Hund, Cat und Ziege.

Kurzübersicht für Vimentin Antikörper (CF®488A) (ABIN6934573)

Target: Vimentin (VIM)

Reaktivität: Human, Schwein, Rind (Kuh), Huhn, Hund, Cat, Ziege

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Vimentin Antikörper ist konjugiert mit CF®488A

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Test (T)

Klon: VM452

Produktdetails anti-Vimentin Antikörper (CF®488A)

Spezifität: This MAb reacts with a 58 kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Anti-vimentin alone is of limited value as a diagnostic tool, however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.

Keine Kreuzreaktivität: Maus, Ratte (Rattus)

Aufreinigung: Purified by Protein A/G

Immunogen: Recombinant human vimentin protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: Jurkat cells, Sarcomas, Melanomas.

Known Application: Flow Cytometry (5 µL per test per one million cells or 5 µL per 100 µL of whole blood),Immunofluorescence (1:50-1:100),Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 100 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA and 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 4 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Vimentin

Target: Vimentin (VIM)

Andere Bezeichnung: VIM

Molekulargewicht: 57-60kDa

Gen-ID: 7431

UniProt: P08670

Pathways: Caspase Kaskade in der Apoptose