EpCAM Antikörper

Produktnummer ABIN6933744

Der Maus Monoklonal anti-EpCAM Antikörper wird verwendet zum Nachweis von EpCAM in Proben von Ratte. Er wurde validiert für WB, FACS, IF, IHC (p), IP und IHC (fro).

Kurzübersicht für EpCAM Antikörper (ABIN6933744)

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser EpCAM Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: SPM534

Produktdetails anti-EpCAM Antikörper

Spezifität: Epitope of GZ-1 and GZ-20 monoclonal antibodies are different. EGP40 is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas.

Keine Kreuzreaktivität: Human

Immunogen: Rat small intestine cells

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: Rat small intestine or colon.

Known Application: Flow Cytometry (5-10 µL/million cells), Immunofluorescence (1:50-1:100), Western Blot (1:100-1:200), Immunoprecipitation (5-10 µL/500 μg protein lysate),Immunohistochemistry (Frozen & formalin-fixed) (1:100-1:200 for 30 min at RT)(Formalin/paraffin tissues REQUIRE digestion of tissue sections with pepsin at 1 mg/mL Tris-HCl, pH 2.0 for 15 min at RT or 10 min at 37C)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Bioreactor Concentrate with 0.05 % Azide. Contact us if you require it in a different format.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu EpCAM

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Andere Bezeichnung: Ep-CAM / CD326

Molekulargewicht: 40-43kDa

Gen-ID: 171577

UniProt: O55159