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Cadherin Antikörper (AA 841-882)

Reaktivität: Human, Maus WB, FACS, IF (cc), IF (p), IHC (p), ELISA, IHC (fro), ICC Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN687682
  • Target Alle Cadherin Produkte
    Cadherin
    Bindungsspezifität
    • 15
    • 8
    • 2
    • 2
    • 1
    • 1
    AA 841-882
    Reaktivität
    • 24
    • 22
    • 7
    • 7
    • 4
    • 3
    • 1
    • 1
    Human, Maus
    Wirt
    • 22
    • 4
    Kaninchen
    Klonalität
    • 21
    • 5
    Polyklonal
    Konjugat
    • 9
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser Cadherin Antikörper ist unkonjugiert
    Applikation
    • 23
    • 14
    • 13
    • 12
    • 7
    • 7
    • 5
    • 3
    • 2
    Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)
    Kreuzreaktivität
    Human, Maus
    Homologie
    Rat,Dog,Cow,Pig,Horse,Chicken
    Aufreinigung
    Purified by Protein A.
    Immunogen
    KLH conjugated synthetic peptide derived from human E-cadherin
    Isotyp
    IgG
  • Applikationshinweise
    WB 1:300-5000
    ELISA 1:500-1000
    FCM 1:20-100
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    ICC 1:100-500
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Validierung #029742 (Flow Cytometry)
    'Independent Validation' Siegel
    by
    Flow Cytometry & Cell Separation Facility, Purdue University
    No.
    #029742
    Datum
    30.06.2014
    Antigen
    Chargennummer
    131101
    Validierte Anwendung
    Flow Cytometry
    Positivkontrolle
    MCF7 cells (high expression)
    Negativkontrolle
    SH-SY5Y cells (low expression)
    Bewertung
    Signal was observed in the positive control cells. There was some non-specific staining observed with the isotype and secondary-only antibody controls which was lower than the signal from the anti-E-cadherin antibody. Low signal was observed in the low expressing (negative control) cells as expected.
    'Independent Validation' Siegel
    Validierungsbilder
    Protokoll
    Primärantikörper
    • Antigen: Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1)
    • Catalog number: ABIN687682
    • Lot number: 131101
    Sekundärantikörper
    • Antibody: Goat anti-rabbit IgG-Alexa 647
    Full Protocol
    • Positive and negative control cells were washed once with phosphate-buffered saline (PBS) and harvested with a non-enzymatic cell dissociation solution (Cellstripper, Mediatech, Inc).
    • Detached cells were washed twice and resuspended in 100 µL 1X PBS containing 0.5% BSA: - unstained cells - secondary antibody alone - 1 µg primary antibody - 1 µg isotype control antibody
    • Cells were incubated for 30 min on ice.
    • Labeled cells were washed twice in PBS containing 0.5% BSA.
    • Cells were resuspended with 1X PBS containing 0.5% BSA + 10% goat serum and incubated for 15 min at room temperature.
    • Goat anti-rabbit IgG-Alexa 647 secondary antibody (Jackson Immunoresearch) was added at a 1:500 dilution. The cells were incubated for 30 min in the dark on ice.
    • Labeled cells were washed twice in PBS containing 0.5% BSA.
    • Propidium Iodide (PI) was added to discern live cells from dead cells.
    • Cells were analyzed on a FACSAria III (BD Biosciences) using a red laser (640 nm excitation / 660 nm emission).
    Anmerkungen
    - The data displayed is gated on PI negative cells.
  • Format
    Liquid
    Konzentration
    1 μg/μL
    Buffer
    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
    Konservierungsmittel
    ProClin
    Vorsichtsmaßnahmen
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    Haltbarkeit
    12 months
  • Yu, Wang, Tang, Zhang, Shang: "RNA interference-mediated knockdown of Notch-1 inhibits migration and invasion, down-regulates matrix metalloproteinases and suppresses NF-?B signaling pathway in trophoblast cells." in: Acta histochemica, Vol. 116, Issue 5, pp. 911-9, (2014) (PubMed).

    Fan, Wang, Zhao, Liu, Chen, Sun, Zheng: "Sonic hedgehog signaling may promote invasion and metastasis of oral squamous cell carcinoma by activating MMP-9 and E-cadherin expression." in: Medical oncology (Northwood, London, England), Vol. 31, Issue 7, pp. 41, (2014) (PubMed).

  • Target
    Cadherin
    Abstract
    Cadherin Produkte
    Synonyme
    ci-cadherin antikoerper, cadherin antikoerper, ci-cadherin antikoerper, LOC430062 antikoerper
    Hintergrund

    Synonyms: UVO, CDHE, ECAD, LCAM, Arc-1, CD324, Cadherin-1, CAM 12/8, Epithelial cadherin, E-cadherin, Uvomorulin, CDH1

    Background: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells, cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.

    Gen-ID
    999
    UniProt
    P12830
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