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Occludin Antikörper (AA 431-522)

OCLN Reaktivität: Human, Maus, Schwein WB, ELISA, FACS, IF (cc), IF (p), IHC (p) Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN687337
  • Target Alle Occludin (OCLN) Antikörper anzeigen
    Occludin (OCLN)
    Bindungsspezifität
    • 16
    • 15
    • 15
    • 9
    • 7
    • 6
    • 5
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 431-522
    Reaktivität
    • 104
    • 65
    • 46
    • 33
    • 18
    • 13
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    Human, Maus, Schwein
    Wirt
    • 86
    • 20
    • 3
    • 1
    Kaninchen
    Klonalität
    • 84
    • 26
    Polyklonal
    Konjugat
    • 46
    • 10
    • 7
    • 6
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    Dieser Occludin Antikörper ist unkonjugiert
    Applikation
    • 88
    • 33
    • 28
    • 25
    • 25
    • 17
    • 15
    • 14
    • 12
    • 8
    • 7
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Kreuzreaktivität
    Human, Maus, Schwein
    Homologie
    Rat,Dog,Cow,Horse,Guinea Pig
    Aufreinigung
    Purified by Protein A.
    Immunogen
    KLH conjugated synthetic peptide derived from mouse Occludin
    Isotyp
    IgG
  • Applikationshinweise
    WB 1:300-5000
    ELISA 1:500-1000
    FCM 1:20-100
    IHC-P 1:200-400
    IF(IHC-P) 1:50-200
    IF(ICC) 1:50-200
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Validierung #029632 (Immunofluorescence)
    'Independent Validation' Siegel
    by
    Confocal Imaging Core, Beth Israel Deaconess Medical Center
    No.
    #029632
    Datum
    23.03.2014
    Antigen
    Chargennummer
    990698W
    Validierte Anwendung
    Immunofluorescence
    Positivkontrolle
    Mouse stomach tissue
    Negativkontrolle
    Human fat tissue
    Bewertung
    Signal was detected in positive control sample and not in negative control sample.
    'Independent Validation' Siegel
    Validierungsbilder
    Protokoll
    Primärantikörper
    • Antibody: Occludin (OCLN)
    • Catalog number: ABIN687337
    • Lot number: 990698W
    Sekundärantikörper
    • Antibody: Donkey anti-Rabbit IgG (Heavy & Light Chain) Antibody (Alexa 647)
    Full Protocol
    • Paraffin embedded positive and negative control tissue sections were deparaffinized and undergone antigen retrieval using 10 mM Sodium Citrate pH6 in a pressure cooker for 10 min.
    • The tissue sections were incubated with 1 mg/mL Sodium borohydride for 10 min at room temperature to block autofluorescent background signal. The sections were then rinsed three times in TBS for 5 min each at RT.
    • Tissue sections were blocked in 1 X TBS / 5% normal donkey serum for 60 min at RT.
    • Tissue sections were incubated with primary antibody diluted 1:200 in 1X PBS / 5% normal donkey serum overnight at 4°C.
    • Tissue sections were rinsed three times in TBS for 5 min each at RT.
    • Cells were incubated with secondary antibody diluted 1:200 in 1X PBS / 5% normal donkey serum for 60 min at RT in dark.
    • Tissue sections were rinsed three times in TBS for 5 min each at RT.
    • Coverslips were mounted on slides with Prolong Gold anti-fade mounting media (Invitrogen)
    • IF stained tissue sections were imaged with a Zeiss LSM 510 Meta confocal microscope.
    Anmerkungen
    • No challenges notes
    • OCLN is recommended to staining of mouse stomach tissue according to this protocol.
  • Format
    Liquid
    Konzentration
    1 μg/μL
    Buffer
    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
    Konservierungsmittel
    ProClin
    Vorsichtsmaßnahmen
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    Haltbarkeit
    12 months
  • Sun, Liu, Xue, Wang: "Effects of insulin combined with idebenone on blood-brain barrier permeability in diabetic rats." in: Journal of neuroscience research, Vol. 93, Issue 4, pp. 666-77, (2015) (PubMed).

    Zhao, Qin, Han, Wang, Zhang, Liu: "?-Conglycinin reduces the tight junction occludin and ZO-1 expression in IPEC-J2." in: International journal of molecular sciences, Vol. 15, Issue 2, pp. 1915-26, (2014) (PubMed).

    Pan, Qin, Zhao, Wang, Liu, Che: "Effects of soybean agglutinin on mechanical barrier function and tight junction protein expression in intestinal epithelial cells from piglets." in: International journal of molecular sciences, Vol. 14, Issue 11, pp. 21689-704, (2013) (PubMed).

    Zhao, Qin, Sun, Che, Bao, Zhang: "Effects of soybean agglutinin on intestinal barrier permeability and tight junction protein expression in weaned piglets." in: International journal of molecular sciences, Vol. 12, Issue 12, pp. 8502-12, (2012) (PubMed).

    Gu, Xue, Wei, Zhang, Li: "Calcium-activated potassium channel activator down-regulated the expression of tight junction protein in brain tumor model in rats." in: Neuroscience letters, Vol. 493, Issue 3, pp. 140-4, (2011) (PubMed).

  • Target
    Occludin (OCLN)
    Andere Bezeichnung
    Occludin (OCLN Produkte)
    Synonyme
    AI503564 antikoerper, Ocl antikoerper, ocln antikoerper, oclnb antikoerper, tpmt antikoerper, OCLN antikoerper, wu:fd23h10 antikoerper, wu:fi13c01 antikoerper, zgc:113992 antikoerper, zgc:56359 antikoerper, BLCPMG antikoerper, occludin antikoerper, occludin S homeolog antikoerper, occludin a antikoerper, Ocln antikoerper, OCLN antikoerper, ocln.S antikoerper, ocln antikoerper, oclna antikoerper
    Hintergrund

    Synonyms: Ocl, AI53564, Occludin, Ocln

    Background: May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier.

    Gen-ID
    18260
    UniProt
    Q61146
    Pathways
    Cell-Cell Junction Organization, Hepatitis C
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