Srebp-1/2 (AA 301-450) Antikörper

Produktnummer ABIN686032

Dieser Kaninchen Polyklonal Antikörper erkennt spezifisch in WB, IF (cc), IF (p), ELISA, IHC (fro) und IHC (p). Er zeigt eine Reaktivität gegenüber Rind (Kuh), Human, Maus und Ratte und wurde in 2+ Publikationen erwähnt.

Kurzübersicht für Srebp-1/2 (AA 301-450) Antikörper (ABIN686032)

Target: Srebp-1/2

Reaktivität: Rind (Kuh), Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails

Bindungsspezifität: AA 301-450

Kreuzreaktivität: Rind (Kuh), Human, Maus, Ratte

Homologie: Sheep,Chicken

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human SREBP-1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Referenzen

Fu, Cui, Zhang: "Lack of ClC-2 Alleviates High Fat Diet-Induced Insulin Resistance and Non-Alcoholic Fatty Liver Disease." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 45, Issue 6, pp. 2187-2198, (2018) (PubMed).

Zhang, Zhang, Chen, Hu, Wang, Jin, Zhang, Wang, Wang, Kang, Li, Li, Pan, Huang, Kong: "Reactive Oxygen Species-Induced TXNIP Drives Fructose-Mediated Hepatic Inflammation and Lipid Accumulation Through NLRP3 Inflammasome Activation." in: Antioxidants & redox signaling, Vol. 22, Issue 10, pp. 848-70, (2015) (PubMed).

Antigendetails

Target: Srebp-1/2

Hintergrund:

Synonyms: Sterol regulatory element binding protein 1, ADD 1, BHLHD1, D630008H06, SREBF 1, SREBF1, SREBP 1, SREBP1, SREBP 1c, Sterol regulatory element binding protein 1, Sterol Regulatory Element Binding Transcription Factor 1 / Protein 1, Sterol regulatory element binding transcription factor 1.

Background: Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway. Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3'). Isoform SREBP-1A is much more active than isoform SREBP-1C in stimulating transcription from SRE-1-containing promoters. [SUBUNIT] Forms a tight complex with SCAP in the ER membrane. Efficient DNA binding of the soluble transcription factor fragment requires dimerization with another bHLH protein. Interacts with LMNA. [SUBCELLULAR LOCATION] Endoplasmic reticulum membrane, Multi-pass membrane protein. Golgi apparatus membrane, Multi-pass membrane protein. Cytoplasmic vesicle, COPII-coated vesicle membrane, Multi-pass membrane protein. Note=Moves from the endoplasmic reticulum to the Golgi in the absence of sterols. [SUBCELLULAR LOCATION] Processed sterol regulatory element-binding protein 1: Nucleus. Belongs to the SREBP family.

Gen-ID: 6720, 6721