LDL Antikörper (PE-Cy7)

Produktnummer ABIN678561

Dieses Anti-LDL-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von LDL in FACS. Geeignet für Human und Maus.

Kurzübersicht für LDL Antikörper (PE-Cy7) (ABIN678561)

Target: LDL (Low Density Lipoprotein (LDL))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LDL Antikörper ist konjugiert mit PE-Cy7

Applikation: Flow Cytometry (FACS)

Produktdetails anti-LDL Antikörper (PE-Cy7)

Kreuzreaktivität: Human

Homologie: Mouse

Aufreinigung: Purified by Protein A.

Immunogen: Full length plasma protein (Human)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: FCM(1:100-500)
Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu LDL

Target: LDL (Low Density Lipoprotein (LDL))

Andere Bezeichnung: LDL

Hintergrund:

Synonyms: _-Lipoprotein, Low density lipoprotein, apolipoprotein B100, apo-B100

Background: LDL and HDL transport both dietary and endogenous cholesterol in the plasma. LDL is the main transporter of cholesterol and cholesteryl esters and makes up more than half of the total lipoprotein in plasma. LDL is absorbed by the liver and other tissues via receptor mediated endocytosis. The cytoplasmic domain of the LDL receptor facilitates the formation of coated pits, receptor-rich regions of the membrane. The ligand binding domain of the receptor recognizes apo-B100 on LDL, resulting in the formation of a clathrin-coated vesicle. ATP-dependent proton pumps lower the pH inside the vesicle resulting dissociation of LDL from its receptor. After loss of the clathrin coat the vesicles fuse with lysozomes, resulting in peptide and cholesteryl ester enzymatic hydrolysis. The LDL receptor can be recycled to the cell membrane. Insulin, tri-iodothyronine and dexamethasome have shown to be involved with the regulation of LDL receptor mediated uptake. The protein component of LDL is apolipoprotein B100. LDL contains 2022 % protein, 1015 % triglycerides, 2028 % phospholipids, 3748 % cholesteryl esters and 810 % cholesterol.