HBxAg Antikörper (HRP)

Produktnummer ABIN678017

Der Kaninchen Polyklonal Anti-HBxAg-Antikörper wurde für WB, ELISA, IHC (fro) und IHC (p) validiert. Er ist geeignet, HBxAg in Proben von Hepatitis B Virus (HBV) zu detektieren.

Kurzübersicht für HBxAg Antikörper (HRP) (ABIN678017)

Target: HBxAg (Hepatitis B Virus X Antigen (HBxAg))

Reaktivität: Hepatitis B Virus (HBV)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HBxAg Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-HBxAg Antikörper (HRP)

Kreuzreaktivität: Virus

Kreuzreaktivität (Details): Hepatitis B virus

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from Hepatitis B virus X protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:100-1000
IHC-P 1:100-500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu HBxAg

Target: HBxAg (Hepatitis B Virus X Antigen (HBxAg))

Andere Bezeichnung: Hepatitis B Virus X Protein

Substanzklasse: Viral Protein

Hintergrund:

Synonyms: HBX, pre-X protein, HB-X, X protein, HBV X protein, X protein [Hepatitis B virus].

Background: Multifunctional protein that may modulate protein degradation pathways, apoptosis, transcription, signal transduction, cell cycle progress, and genetic stability by directly or indirectly interacting with hosts factors. Does not seem to be essential for HBV infection. May be directly involved in development of cirrhosis and liver cancer (hepatocellular carcinoma). Most of cytosolic activities involve modulation of cytosolic calcium. The effect on apoptosis is controversial depending on the cell types in which the studies have been conducted. By binding to human DDB1, may affect cell viability and stimulate genome replication. May induce apoptosis by localizing in mitochondria and causing loss of mitochondrial membrane potential. May also modulate apoptosis by binding human CFLAR, a key regulator of the death-inducing signaling complex (DISC). Moderately stimulates transcription of many different viral and cellular transcription elements. Promoters and enhancers stimulated by HBx contain DNA binding sites for NF-kappa-B, AP-1, AP-2, c-EBP, ATF/CREB, or the calcium-activated factor NF-AT. May bind bZIP transcription factors like CREB1 (By similarity).