Sialylated Mucin-Type-O-Glycans Antikörper

Produktnummer ABIN6757949

Produktdetails

Target: Sialylated Mucin-Type-O-Glycans

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Spezifität: Sialylated Mucin-Type-O-Glycans.

Aufreinigung: Purified

Immunogen: Purified mucus-glycoproteins from human amniotic fluid.

Klon: FW17

Isotyp: IgM

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, ICC, IHC, IHC-P

Usage: Tumor and normal tissues were first fixed in 6 % neutral phosphate-buffered formalin and dehydrated in ethanol by standard histological techniques. After embedding all tissues in paraffin, serial microtome tissue sections (5 μm) were used for comparative immunohistological studies. Reactivity of hybridoma tissue culture supernatants was determined using a four-step immunoperoxidase technique. After deparaffinization in xylene, rehydration through graded ethanol, and blocking endogenous peroxidase activity with 2 % H202 in absolute methanol for 30 min, the sections were incubated as follows: (1) normal swine serum (5 % in PBS), (2) MAb in tissue culture supernatant or mouse ascites fluid diluted to 1/100 in PBS, (3) rabbit anti-mouse immunoglobulin (2 % in PBS), (4) swine anti-rabbit immunoglobulin (2 % in PBS), (5) rabbit peroxidase anti-peroxidase (PAP) complex. The slides were treated with 0.2 mg/mL - ' 3-amino-9-ethylcarbazole in 0.02 M sodium acetate buffer containing 0.01 % H202, then counterstained with hematoxylin, and mounted in glycerol jelly. All incubation steps were performed in a moist chamber at 21 °C for 30 min.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Lyophilized powder may be stored at 4°C for short-term only. Reconstituted product is stable for 1 year at -20°C.

Antigendetails

Target: Sialylated Mucin-Type-O-Glycans