CPXCR1 Antikörper (AA 396-445)

Produktnummer ABIN6744465

Der Kaninchen Polyklonal Anti-CPXCR1-Antikörper wurde für WB, IHC und IHC (p) validiert. Er ist geeignet, CPXCR1 in Proben von Human zu detektieren.

Kurzübersicht für CPXCR1 Antikörper (AA 396-445) (ABIN6744465)

Target: CPXCR1 (CPX Chromosome Region, Candidate 1 (CPXCR1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CPXCR1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-CPXCR1 Antikörper

Bindungsspezifität: AA 396-445

Marke: IHC-plus™

Spezifität: Human CPXCR1

Homologie: Percent identity by BLAST analysis: Human (100%) Monkey (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa396-445 of human CPXCR1 (Q8N123, NP_149037). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla (100%), Gibbon, Monkey (85%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (10 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu CPXCR1

Target: CPXCR1 (CPX Chromosome Region, Candidate 1 (CPXCR1))

Andere Bezeichnung: CPXCR1 / CT77

Hintergrund: Name/Gene ID: CPXCR1

Synonyms: CPXCR1, Cancer/testis antigen 77, CT77

Gen-ID: 53336

NCBI Accession: NP_149037

UniProt: Q8N123