KIF20A Antikörper (C-Term)

Produktnummer ABIN6744463

Dieses Anti-KIF20A-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von KIF20A in WB, IHC und IHC (p). Geeignet für Human, Ratte, Hund, Pferd, Schwein, Kaninchen und Rind (Kuh).

Kurzübersicht für KIF20A Antikörper (C-Term) (ABIN6744463)

Target: KIF20A (Kinesin Family Member 20A (KIF20A))

Reaktivität: Human, Ratte, Hund, Pferd, Schwein, Kaninchen, Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser KIF20A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-KIF20A Antikörper

Bindungsspezifität: C-Term

Marke: IHC-plus™

Spezifität: Human KIF20A

Homologie: Percent identity by BLAST analysis: Human, Dog, Rabbit, Horse, Pig (100%) Chicken (92%) Rat (86%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human KIF20A (O95235, NP_005724). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Elephant, Panda, Dog, Bovine, Rabbit, Horse, Pig, Opossum (100%), Galago, Marmoset, Mouse, Bat, Guinea pig (93%), Turkey, Chicken (92%), Rat (86%), Zebra finch (84%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute with 50 μL sterile ddH2O.

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu KIF20A

Target: KIF20A (Kinesin Family Member 20A (KIF20A))

Andere Bezeichnung: RAB6KIFL / KIF20A

Hintergrund: Name/Gene ID: KIF20A

Synonyms: KIF20A, Kinesin family member 20A, Kinesin-like protein KIF20A, GG10_2, Mitotic kinesin-like protein 2, MKLP2, Rabkinesin-6, RAB6KIFL

Gen-ID: 10112

NCBI Accession: NP_005724

UniProt: O95235