FOXM1 Antikörper (C-Term)

Produktnummer ABIN490076

Der Kaninchen Polyklonal Anti-FOXM1-Antikörper wurde für WB, IHC und IHC (p) validiert. Er ist geeignet, FOXM1 in Proben von Human, Hund, Meerschweinchen und Schwein zu detektieren.

Kurzübersicht für FOXM1 Antikörper (C-Term) (ABIN490076)

Target: FOXM1 (Forkhead Box M1 (FOXM1))

Reaktivität: Human, Hund, Meerschweinchen, Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser FOXM1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-FOXM1 Antikörper

Bindungsspezifität: C-Term

Marke: IHC-plus™

Spezifität: Human FOXM1

Homologie: Percent identity by BLAST analysis: Human (100%) Mouse, Rat, Rabbit (91%) Dog, Pig, Guinea pig (83%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human FOXM1 (Q08050, NP_068772). Percent identity by BLAST analysis: Human, Chimpanzee (100%), Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Rabbit (91%), Elephant (90%), Galago, Panda, Dog, Pig, Guinea pig (83%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu FOXM1

Target: FOXM1 (Forkhead Box M1 (FOXM1))

Andere Bezeichnung: FOXM1

Hintergrund: Name/Gene ID: FOXM1
Family: Transcription factor

Synonyms: FOXM1, Forkhead box M1, Forkhead box protein M1, HFH11, HNF-3, HNF-3/fork-head homolog 11, INS-1, FOXM1B, M-phase phosphoprotein 2, MPP-2, MPHOSPH2, PIG29, TRIDENT, FKHL16, HFH-11, TGT3, Transcription factor Trident, WIN

Gen-ID: 2305

NCBI Accession: NP_068772

UniProt: Q08050

Pathways: Positive Regulation of Response to DNA Damage Stimulus