Septin 11 Antikörper (N-Term)

Produktnummer ABIN6744459

Der Kaninchen Polyklonal Anti-Septin 11-Antikörper wurde für WB, IHC und IHC (p) validiert. Er ist geeignet, Septin 11 in Proben von Human, Maus, Ratte, Pferd, Rind (Kuh), Meerschweinchen, Kaninchen, Hund, Affe und Schwein zu detektieren.

Kurzübersicht für Septin 11 Antikörper (N-Term) (ABIN6744459)

Target: Septin 11 (SEPT11)

Reaktivität: Human, Maus, Ratte, Pferd, Rind (Kuh), Meerschweinchen, Kaninchen, Hund, Affe, Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Septin 11 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Septin 11 Antikörper

Bindungsspezifität: N-Term

Marke: IHC-plus™

Spezifität: Human SEPT11 / Septin 11

Homologie: Percent identity by BLAST analysis: Human, Orangutan, Monkey, Mouse, Rat, Bovine (100%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human SEPT11 (Q9NVA2, NP_060713). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig (100%), Opossum (85%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu Septin 11

Target: Septin 11 (SEPT11)

Andere Bezeichnung: SEPT11 / Septin 11

Hintergrund: Name/Gene ID: SEPT11

Synonyms: SEPT11, Septin 11, Septin-11

Gen-ID: 55752

NCBI Accession: NP_060713

UniProt: Q9NVA2