SH3BP2 Antikörper (AA 432-481)

Produktnummer ABIN6744458

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch SH3BP2 in WB, IHC und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Affe und Rind (Kuh).

Kurzübersicht für SH3BP2 Antikörper (AA 432-481) (ABIN6744458)

Target: SH3BP2 (SH3-Domain Binding Protein 2 (SH3BP2))

Reaktivität: Human, Maus, Ratte, Affe, Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SH3BP2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-SH3BP2 Antikörper

Bindungsspezifität: AA 432-481

Marke: IHC-plus™

Spezifität: Human SH3BP2

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat (100%) Rabbit, Guinea pig (92%) Bovine (84%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa432-481 of human SH3BP2 (P78314, NP_003014). Percent identity by BLAST analysis: Human, Gorilla, Marmoset, Mouse, Rat, Opossum (100%), Elephant, Bat, Rabbit, Guinea pig (92%), Galago (85%), Bovine (84%), Zebra finch (83%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu SH3BP2

Target: SH3BP2 (SH3-Domain Binding Protein 2 (SH3BP2))

Andere Bezeichnung: SH3BP2

Hintergrund: Name/Gene ID: SH3BP2

Synonyms: SH3BP2, Abl-SH3 binding protein 2, 3BP-2, 3BP2, Cherubism, CRPM, RES4-23, SH3 domain-binding protein 2, TNFAIP3 interacting protein 2, CRBM, SH3-domain binding protein 2

Gen-ID: 6452

NCBI Accession: NP_003014

UniProt: P78314

Pathways: T-Zell Rezeptor Signalweg