FUBP3 Antikörper (AA 503-552)

Produktnummer ABIN6744454

Der Kaninchen Polyklonal Anti-FUBP3-Antikörper wurde für WB, IHC und IHC (p) validiert. Er ist geeignet, FUBP3 in Proben von Human, Maus, Ratte, Meerschweinchen und Hund zu detektieren.

Kurzübersicht für FUBP3 Antikörper (AA 503-552) (ABIN6744454)

Target: FUBP3 (Far Upstream Element (FUSE) Binding Protein 3 (FUBP3))

Reaktivität: Human, Maus, Ratte, Meerschweinchen, Hund

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser FUBP3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-FUBP3 Antikörper

Bindungsspezifität: AA 503-552

Marke: IHC-plus™

Spezifität: Human FUBP3 / FBP3

Homologie: Percent identity by BLAST analysis: Human (100%) Bovine, Horse, Pig (92%) Mouse, Rat, Dog (85%) Guinea pig (84%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa503-552 of human FUBP3 (Q96I24, NP_003925). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon (100%), Monkey, Galago, Marmoset, Elephant, Bovine, Bat, Horse, Pig (92%), Mouse, Rat, Dog (85%), Guinea pig (84%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu FUBP3

Target: FUBP3 (Far Upstream Element (FUSE) Binding Protein 3 (FUBP3))

Andere Bezeichnung: FUBP3

Hintergrund: Name/Gene ID: FUBP3

Synonyms: FUBP3, FBP3, FUSE-binding protein 3

Gen-ID: 8939

NCBI Accession: NP_003925

UniProt: Q96I24