HMGCS1 Antikörper (AA 251-300)

Produktnummer ABIN6744452

Produktdetails anti-HMGCS1 Antikörper

Target: HMGCS1 (3-Hydroxy-3-Methylglutaryl-CoA Synthase 1 (Soluble) (HMGCS1))

Bindungsspezifität: AA 251-300

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Pferd, Kaninchen, Schwein, Hamster, Xenopus laevis

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HMGCS1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human HMGCS1

Homologie: Percent identity by BLAST analysis: Human, Mouse, Dog, Bovine, Rabbit, Horse, Pig (100%) Guinea pig (92%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa251-300 of human HMGCS1 (Q01581, NP_002121). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Xenopus (100%), Marmoset, Bat, Guinea pig (92%), Opossum (85%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu HMGCS1

Target: HMGCS1 (3-Hydroxy-3-Methylglutaryl-CoA Synthase 1 (Soluble) (HMGCS1))

Andere Bezeichnung: HMGCS1 / HMG-CoA Synthase 1 (HMGCS1 Produkte)

Synonyme: HMGCS antikoerper, zgc:56481 antikoerper, B130032C06Rik antikoerper, 3-hydroxy-3-methylglutaryl-CoA synthase 1 antikoerper, 3-hydroxy-3-methylglutaryl-CoA synthase 1 (soluble) antikoerper, 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1 antikoerper, 3-hydroxy-3-methylglutaryl coenzyme A synthase antikoerper, hydroxymethylglutaryl-CoA synthase antikoerper, 3-hydroxy-3-methylglutaryl-CoA synthase antikoerper, hydroxymethylglutaryl coenzyme A synthase antikoerper, 3-hydroxy-3-methylglutaryl-CoA synthase 1 S homeolog antikoerper, HMGCS1 antikoerper, hmgcs1 antikoerper, Hmgcs1 antikoerper, mvaS antikoerper, SAS2432 antikoerper, SZO_RS04705 antikoerper, SEQ_1109 antikoerper, NAEGRDRAFT_77778 antikoerper, hmgcs1.S antikoerper

Hintergrund: Name/Gene ID: HMGCS1

Synonyms: HMGCS1, HMG-CoA synthase, HMGCS

Gen-ID: 3157

NCBI Accession: NP_002121

UniProt: Q01581

Pathways: Regulation of Lipid Metabolism by PPARalpha