HMGCS1 Antikörper (AA 251-300)

Produktnummer ABIN6744452

Dieses Anti-HMGCS1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von HMGCS1 in WB, IHC und IHC (p). Geeignet für Human, Maus, Ratte, Rind (Kuh), Hund, Pferd, Kaninchen, Schwein, Hamster und Xenopus laevis.

Kurzübersicht für HMGCS1 Antikörper (AA 251-300) (ABIN6744452)

Target: HMGCS1 (3-Hydroxy-3-Methylglutaryl-CoA Synthase 1 (Soluble) (HMGCS1))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Pferd, Kaninchen, Schwein, Hamster, Xenopus laevis

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HMGCS1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-HMGCS1 Antikörper

Bindungsspezifität: AA 251-300

Marke: IHC-plus™

Spezifität: Human HMGCS1

Homologie: Percent identity by BLAST analysis: Human, Mouse, Dog, Bovine, Rabbit, Horse, Pig (100%) Guinea pig (92%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa251-300 of human HMGCS1 (Q01581, NP_002121). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Xenopus (100%), Marmoset, Bat, Guinea pig (92%), Opossum (85%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu HMGCS1

Target: HMGCS1 (3-Hydroxy-3-Methylglutaryl-CoA Synthase 1 (Soluble) (HMGCS1))

Andere Bezeichnung: HMGCS1 / HMG-CoA Synthase 1

Hintergrund: Name/Gene ID: HMGCS1

Synonyms: HMGCS1, HMG-CoA synthase, HMGCS

Gen-ID: 3157

NCBI Accession: NP_002121

UniProt: Q01581

Pathways: Regulation of Lipid Metabolism by PPARalpha