CBP Antikörper (AA 144-193)

Produktnummer ABIN6744446

Der Kaninchen Polyklonal Anti-CBP-Antikörper wurde für WB, IHC und IHC (p) validiert. Er ist geeignet, CBP in Proben von Human, Maus, Ratte, Rind (Kuh), Hund, Kaninchen, Huhn, Meerschweinchen und Pferd zu detektieren.

Kurzübersicht für CBP Antikörper (AA 144-193) (ABIN6744446)

Target: CBP (CREBBP) (CREB Binding Protein (CREBBP))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Kaninchen, Huhn, Meerschweinchen, Pferd

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CBP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-CBP Antikörper

Bindungsspezifität: AA 144-193

Marke: IHC-plus™

Spezifität: Human CREBBP

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Guinea pig, Chicken (100%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa144-193 of human CREBBP (Q4LE28, NP_001073315). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Mouse, Rat, Elephant, Panda, Dog, Bovine, Rabbit, Horse, Opossum, Guinea pig, Turkey, Zebra finch, Chicken (100%), Marmoset (93%), Platypus (85%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu CBP

Target: CBP (CREBBP) (CREB Binding Protein (CREBBP))

Andere Bezeichnung: CREBBP / CBP

Hintergrund: Name/Gene ID: CREBBP

Synonyms: CREBBP, CREB binding protein, CREB-binding protein, KAT3A, RSTS, Rubinstein-Taybi syndrome, RTS, CBP

Gen-ID: 1387

NCBI Accession: NP_001073315

UniProt: Q92793

Pathways: T-Zell Rezeptor Signalweg, Interferon-gamma Pathway, Stem Cell Maintenance, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha