ZNF71 Antikörper (C-Term)

Produktnummer ABIN6744444

Der Kaninchen Polyklonal Anti-ZNF71-Antikörper wurde für WB, IHC und IHC (p) validiert. Er ist geeignet, ZNF71 in Proben von Human, Pferd und Affe zu detektieren.

Kurzübersicht für ZNF71 Antikörper (C-Term) (ABIN6744444)

Target: ZNF71 (Zinc Finger Protein 71 (ZNF71))

Reaktivität: Human, Pferd, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ZNF71 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-ZNF71 Antikörper

Bindungsspezifität: C-Term

Marke: IHC-plus™

Spezifität: Human ZNF71

Homologie: Percent identity by BLAST analysis: Human, Horse (100%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human ZNF71 (Q9NQZ8, NP_067039). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Galago, Marmoset, Horse (100%), Gibbon, Mouse, Rat, Bovine, Bat, Guinea pig, Drosophila (85%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu ZNF71

Target: ZNF71 (Zinc Finger Protein 71 (ZNF71))

Andere Bezeichnung: ZNF71

Hintergrund: Name/Gene ID: ZNF71
Family: Zinc Finger

Synonyms: ZNF71, Cos26, Zinc finger protein 71, Zinc finger protein 71 (Cos26), EZFIT

Gen-ID: 58491

NCBI Accession: NP_067039

UniProt: Q9NQZ8