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PHACTR1 Antikörper (AA 468-517)

PHACTR1 Reaktivität: Human, Maus, Ratte, Rind (Kuh), Meerschweinchen, Pferd, Kaninchen, Huhn, Affe, Schwein, Xenopus laevis WB, IHC, IHC (p) Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN6744441
  • Target Alle PHACTR1 Antikörper anzeigen
    PHACTR1 (Phosphatase and Actin Regulator 1 (PHACTR1))
    Bindungsspezifität
    • 4
    • 1
    AA 468-517
    Reaktivität
    Human, Maus, Ratte, Rind (Kuh), Meerschweinchen, Pferd, Kaninchen, Huhn, Affe, Schwein, Xenopus laevis
    Wirt
    • 8
    Kaninchen
    Klonalität
    • 8
    Polyklonal
    Konjugat
    • 5
    • 1
    • 1
    • 1
    Dieser PHACTR1 Antikörper ist unkonjugiert
    Applikation
    • 5
    • 4
    • 2
    • 2
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Marke
    IHC-plus™
    Spezifität
    Human PHACTR1
    Homologie
    Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Horse, Chicken (100%).
    Aufreinigung
    Immunoaffinity purified
    Immunogen
    Synthetic peptide located between aa468-517 of human PHACTR1 (Q9C0D0, NP_112210). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Rat, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Zebra finch, Chicken, Xenopus (100%), Turkey (81%).

    Type of Immunogen: Synthetic peptide
    Isotyp
    IgG
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  • Applikationshinweise
    Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.
    Kommentare

    Target Species of Antibody: Human

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Distilled water
    Konzentration
    Lot specific
    Buffer
    Lyophilized from PBS with 2 % sucrose
    Handhabung
    Avoid repeat freeze-thaw cycles.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • Target
    PHACTR1 (Phosphatase and Actin Regulator 1 (PHACTR1))
    Andere Bezeichnung
    PHACTR1 (PHACTR1 Produkte)
    Synonyme
    DDSG1 antikoerper, RPEL antikoerper, RPEL1 antikoerper, dJ257A7.2 antikoerper, 9630030F18Rik antikoerper, Rpel1 antikoerper, phosphatase and actin regulator 1 S homeolog antikoerper, phosphatase and actin regulator 1 antikoerper, phactr1.S antikoerper, PHACTR1 antikoerper, Phactr1 antikoerper, LOC100726748 antikoerper
    Hintergrund
    Name/Gene ID: PHACTR1

    Synonyms: PHACTR1, DJ257A7.2, RPEL1, RPEL, RPEL repeat containing 1, KIAA1733
    Gen-ID
    221692
    NCBI Accession
    NP_112210
    UniProt
    Q9C0D0
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