PHACTR1 Antikörper (AA 468-517)

Produktnummer ABIN6744441

Der Kaninchen Polyklonal Anti-PHACTR1-Antikörper wurde für WB, IHC und IHC (p) validiert. Er ist geeignet, PHACTR1 in Proben von Human, Maus, Ratte, Rind (Kuh), Meerschweinchen, Pferd, Kaninchen, Huhn, Affe, Schwein und Xenopus laevis zu detektieren.

Kurzübersicht für PHACTR1 Antikörper (AA 468-517) (ABIN6744441)

Target: PHACTR1 (Phosphatase and Actin Regulator 1 (PHACTR1))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Meerschweinchen, Pferd, Kaninchen, Huhn, Affe, Schwein, Xenopus laevis

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PHACTR1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-PHACTR1 Antikörper

Bindungsspezifität: AA 468-517

Marke: IHC-plus™

Spezifität: Human PHACTR1

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Horse, Chicken (100%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa468-517 of human PHACTR1 (Q9C0D0, NP_112210). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Rat, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Zebra finch, Chicken, Xenopus (100%), Turkey (81%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu PHACTR1

Target: PHACTR1 (Phosphatase and Actin Regulator 1 (PHACTR1))

Andere Bezeichnung: PHACTR1

Hintergrund: Name/Gene ID: PHACTR1

Synonyms: PHACTR1, DJ257A7.2, RPEL1, RPEL, RPEL repeat containing 1, KIAA1733

Gen-ID: 221692

NCBI Accession: NP_112210

UniProt: Q9C0D0