ZNF777 Antikörper (AA 468-517)

Produktnummer ABIN6741624

Produktdetails anti-ZNF777 Antikörper

Target: ZNF777 (Zinc Finger Protein 777 (ZNF777))

Bindungsspezifität: AA 468-517

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Meerschweinchen, Pferd, Kaninchen, Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ZNF777 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human ZNF777

Homologie: Percent identity by BLAST analysis: Human, Bovine, Rabbit, Horse, Pig, Guinea pig (100%) Dog (92%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa468-517 of human ZNF777 (Q9ULD5, NP_056509). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey, Galago, Mouse, Rat, Bovine, Rabbit, Horse, Pig, Guinea pig (100%), Gorilla, Marmoset, Dog, Bat (92%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:12500.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu ZNF777

Target: ZNF777 (Zinc Finger Protein 777 (ZNF777))

Andere Bezeichnung: ZNF777 (ZNF777 Produkte)

Synonyme: 2500002G23Rik antikoerper, ZNF777 antikoerper, RGD1566056 antikoerper, Znf777 antikoerper, zinc finger protein 777 antikoerper, Zfp777 antikoerper, ZNF777 antikoerper, LOC769049 antikoerper

Hintergrund: Name/Gene ID: ZNF777
Family: Zinc Finger

Synonyms: ZNF777, Zinc finger protein 777, KIAA1285

Gen-ID: 27153

NCBI Accession: NP_056509

UniProt: Q9ULD5