EGR2 Antikörper (AA 397-446)

Produktnummer ABIN6741623

Der Kaninchen Polyklonal Anti-EGR2-Antikörper wurde für WB, IHC und IHC (p) validiert. Er ist geeignet, EGR2 in Proben von Human, Maus, Pferd, Rind (Kuh) und Affe zu detektieren.

Kurzübersicht für EGR2 Antikörper (AA 397-446) (ABIN6741623)

Target: EGR2 (Early Growth Response 2 (EGR2))

Reaktivität: Human, Maus, Pferd, Rind (Kuh), Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EGR2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-EGR2 Antikörper

Bindungsspezifität: AA 397-446

Marke: IHC-plus™

Spezifität: Human EGR2

Homologie: Percent identity by BLAST analysis: Human, Mouse, Bovine (100%) Rat, Dog, Rabbit, Pig (92%) Horse (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa397-446 of human EGR2 (P11161, NP_000390). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Bovine (100%), Rat, Hamster, Elephant, Panda, Dog, Rabbit, Pig, Guinea pig (92%), Galago, Horse (85%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS buffer with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu EGR2

Target: EGR2 (Early Growth Response 2 (EGR2))

Andere Bezeichnung: EGR2

Hintergrund: Name/Gene ID: EGR2
Family: Transcription factor

Synonyms: EGR2, CMT1D, CMT4E, Early growth response 2, E3 SUMO-protein ligase EGR2, EGR-2, KROX20, Krox-20, AT591, Zinc finger protein Krox-20

Gen-ID: 1959

NCBI Accession: NP_000390

UniProt: P11161