IMPDH1 Antikörper (AA 254-303)

Produktnummer ABIN6741618

Dieses Anti-IMPDH1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von IMPDH1 in WB, IHC und IHC (p). Geeignet für Human, Maus, Ratte, Rind (Kuh), Hund, Meerschweinchen, Pferd, Zebrafisch (Danio rerio), Fledermaus, Affe, Schwein, Xenopus laevis, Huhn und Hamster.

Kurzübersicht für IMPDH1 Antikörper (AA 254-303) (ABIN6741618)

Target: IMPDH1 (Inosine 5'-Phosphate Dehydrogenase 1 (IMPDH1))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Meerschweinchen, Pferd, Zebrafisch (Danio rerio), Fledermaus, Affe, Schwein, Xenopus laevis, Huhn, Hamster

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IMPDH1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-IMPDH1 Antikörper

Bindungsspezifität: AA 254-303

Marke: IHC-plus™

Spezifität: Human IMPDH1

Homologie: Percent identity by BLAST analysis: Human, Xenopus, Zebrafish (100%) Mouse, Dog, Rabbit, Horse, Pig, Guinea pig (92%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa254-303 of human IMPDH1 (P20839, NP_899066). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Bat, Horse, Pig, Opossum, Guinea pig, Turkey, Chicken, Platypus, Lizard, Xenopus, Salmon, Zebrafish (100%), Rabbit, Stickleback (92%), Toxoplasma (84%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled Water.

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu IMPDH1

Target: IMPDH1 (Inosine 5'-Phosphate Dehydrogenase 1 (IMPDH1))

Andere Bezeichnung: IMPDH1

Hintergrund: Name/Gene ID: IMPDH1

Synonyms: IMPDH1, IMP dehydrogenase 1, IMPD 1, IMPDH 1, IMPD1, LCA11, IMPD, IMPDH-I, SWSS2608, RP10

Gen-ID: 3614

NCBI Accession: NP_899066

UniProt: P20839

Pathways: Ribonucleoside Biosynthetic Process