MAS1 Antikörper (AA 187-236)

Produktnummer ABIN6741611

Produktdetails anti-MAS1 Antikörper

Target: MAS1 (MAS1 Oncogene (MAS1))

Bindungsspezifität: AA 187-236

Reaktivität: Human, Maus, Ratte, Hamster, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MAS1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human MAS1

Homologie: Percent identity by BLAST analysis: Mouse (100%) Bovine (92%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa187-236 of human MAS1 (P04201, NP_002368). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Mouse, Rat, Hamster, Dolphin (100%), Bovine (92%), Panda, Opossum, Guinea pig (85%), Galago (84%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 0.25 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled Water.

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu MAS1

Target: MAS1 (MAS1 Oncogene (MAS1))

Andere Bezeichnung: MAS1 / MAS (MAS1 Produkte)

Synonyme: MAS antikoerper, Mas-1 antikoerper, c-mas antikoerper, MAS1 antikoerper, MAS1 proto-oncogene, G protein-coupled receptor antikoerper, MAS1 oncogene antikoerper, proto-oncogene Mas antikoerper, MAS1 antikoerper, Mas1 antikoerper, LOC703105 antikoerper

Hintergrund: Name/Gene ID: MAS1
Subfamily: Orphan-A
Family: GPCR

Synonyms: MAS1, Mas proto-oncogene, Proto-oncogene Mas, MAS, MAS1 oncogene

Gen-ID: 4142

NCBI Accession: NP_002368

UniProt: P04201

Pathways: ACE Inhibitor Pathway, Regulation of Carbohydrate Metabolic Process