ARID5A Antikörper (C-Term)

Produktnummer ABIN6741610

Produktdetails anti-ARID5A Antikörper

Target: ARID5A (AT Rich Interactive Domain 5A (MRF1-Like) (ARID5A))

Bindungsspezifität: C-Term

Reaktivität: Human, Maus, Ratte, Hund, Meerschweinchen, Kaninchen, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ARID5A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human ARID5A

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Rabbit (100%) Pig (93%) Horse (91%) Guinea pig (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human ARID5A (Q03989, NP_997646). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Mouse, Rat, Elephant, Dog, Rabbit (100%), Bat, Pig (93%), Panda, Horse (91%), Marmoset, Guinea pig (85%), Bovine (80%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled Water.

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu ARID5A

Target: ARID5A (AT Rich Interactive Domain 5A (MRF1-Like) (ARID5A))

Andere Bezeichnung: MRF-1 / ARID5A (ARID5A Produkte)

Hintergrund: Name/Gene ID: ARID5A

Synonyms: ARID5A, Modulator recognition factor 1, MRF-1, MRF1, RFVG5814, RP11-363D14, Modulator recognition factor I

Gen-ID: 10865

NCBI Accession: NP_997646

UniProt: Q03989