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ARID5A Antikörper (C-Term)

Dieses Anti-ARID5A-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von ARID5A in WB, IHC und IHC (p). Geeignet für Human, Maus, Ratte, Hund, Meerschweinchen, Kaninchen und Affe.
Produktnummer ABIN6741610

Kurzübersicht für ARID5A Antikörper (C-Term) (ABIN6741610)

Target

Alle ARID5A Antikörper anzeigen
ARID5A (AT Rich Interactive Domain 5A (MRF1-Like) (ARID5A))

Reaktivität

  • 23
  • 7
  • 6
  • 5
  • 4
  • 3
  • 3
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
Human, Maus, Ratte, Hund, Meerschweinchen, Kaninchen, Affe

Wirt

  • 18
  • 5
Kaninchen

Klonalität

  • 20
  • 3
Polyklonal

Konjugat

  • 18
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser ARID5A Antikörper ist unkonjugiert

Applikation

  • 22
  • 11
  • 3
  • 2
  • 2
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • Bindungsspezifität

    • 7
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term

    Marke

    IHC-plus™

    Spezifität

    Human ARID5A

    Homologie

    Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Rabbit (100%) Pig (93%) Horse (91%) Guinea pig (85%).

    Aufreinigung

    Immunoaffinity purified

    Immunogen

    Synthetic peptide from C-Terminus of human ARID5A (Q03989, NP_997646). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Mouse, Rat, Elephant, Dog, Rabbit (100%), Bat, Pig (93%), Panda, Horse (91%), Marmoset, Guinea pig (85%), Bovine (80%).

    Type of Immunogen: Synthetic peptide

    Isotyp

    IgG
  • Applikationshinweise

    Approved: IHC, IHC-P (5 μg/mL), WB

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

    Kommentare

    Target Species of Antibody: Human

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Distilled Water.

    Konzentration

    Lot specific

    Buffer

    Lyophilized from PBS with 2 % sucrose

    Handhabung

    Avoid repeat freeze-thaw cycles.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • Target

    ARID5A (AT Rich Interactive Domain 5A (MRF1-Like) (ARID5A))

    Andere Bezeichnung

    MRF-1 / ARID5A

    Hintergrund

    Name/Gene ID: ARID5A

    Synonyms: ARID5A, Modulator recognition factor 1, MRF-1, MRF1, RFVG5814, RP11-363D14, Modulator recognition factor I

    Gen-ID

    10865

    NCBI Accession

    NP_997646

    UniProt

    Q03989
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