SLC35A5 Antikörper (AA 36-85)

Produktnummer ABIN6741602

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch SLC35A5 in WB, IHC und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Rind (Kuh), Hund, Meerschweinchen, Pferd, Fledermaus, Affe und Schwein.

Kurzübersicht für SLC35A5 Antikörper (AA 36-85) (ABIN6741602)

Target: SLC35A5 (Solute Carrier Family 35, Member A5 (SLC35A5))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Meerschweinchen, Pferd, Fledermaus, Affe, Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SLC35A5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-SLC35A5 Antikörper

Bindungsspezifität: AA 36-85

Marke: IHC-plus™

Spezifität: Human SLC35A5

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Horse, Pig, Guinea pig (100%) Rabbit, Chicken (92%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa36-85 of human SLC35A5 (Q9BS91, NP_060415). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Horse, Pig, Guinea pig, Platypus (100%), Rabbit, Turkey, Zebra finch, Chicken, Lizard (92%), Opossum (85%), Zebrafish (84%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled Water.

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu SLC35A5

Target: SLC35A5 (Solute Carrier Family 35, Member A5 (SLC35A5))

Andere Bezeichnung: SLC35A5

Hintergrund: Name/Gene ID: SLC35A5
Subfamily: Drμg/metabolite transporter
Family: Transporter

Synonyms: SLC35A5

Gen-ID: 55032

NCBI Accession: NP_060415

UniProt: Q9BS91