GTPBP10 Antikörper (AA 254-303)

Produktnummer ABIN6741598

Dieses Anti-GTPBP10-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von GTPBP10 in WB, IHC und IHC (p). Geeignet für Human, Maus, Ratte, Kaninchen, Pferd, Schwein, Hamster und Affe.

Kurzübersicht für GTPBP10 Antikörper (AA 254-303) (ABIN6741598)

Target: GTPBP10 (GTP-Binding Protein 10 (GTPBP10))

Reaktivität: Human, Maus, Ratte, Kaninchen, Pferd, Schwein, Hamster, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GTPBP10 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-GTPBP10 Antikörper

Bindungsspezifität: AA 254-303

Marke: IHC-plus™

Spezifität: Human GTPBP10

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Rabbit, Horse, Pig (100%) Dog, Guinea pig (92%) Chicken (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa254-303 of human GTPBP10 (A4D1E9, NP_149098). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Baboon, Monkey, Galago, Marmoset, Mouse, Rat, Shrew, Hamster, Elephant, Rabbit, Horse, Pig, Opossum, Armadillo, Lizard, Stickleback (100%), Dog, Bovine, Bat, Guinea pig (92%), Salmon (90%), Turkey, Zebra finch, Chicken, Platypus (85%), Zebrafish (84%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled Water.

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu GTPBP10

Target: GTPBP10 (GTP-Binding Protein 10 (GTPBP10))

Andere Bezeichnung: GTPBP10

Hintergrund: Name/Gene ID: GTPBP10

Synonyms: GTPBP10, DKFZP686A10121, ObgH2, Protein obg homolog 2, UG0751c10, GTP-binding protein 10

Gen-ID: 85865

NCBI Accession: NP_149098

UniProt: A4D1E9