LIN7C Antikörper (N-Term)

Produktnummer ABIN6741597

Produktdetails anti-LIN7C Antikörper

Target: LIN7C (Lin-7 Homolog C (LIN7C))

Bindungsspezifität: N-Term

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Meerschweinchen, Pferd, Kaninchen, Fledermaus, Huhn, Hamster, Affe, Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LIN7C Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human LIN7C

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Chicken (100%) Zebrafish (92%) Xenopus (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human LIN7C (Q9NUP9, NP_060832). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard (100%), Salmon, Stickleback, Zebrafish (92%), Xenopus, Smelt (85%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled Water.

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu LIN7C

Target: LIN7C (Lin-7 Homolog C (LIN7C))

Andere Bezeichnung: LIN7C / VELI3 (LIN7C Produkte)

Synonyme: LIN7C antikoerper, fb75b09 antikoerper, wu:fb75b09 antikoerper, zgc:101881 antikoerper, LIN-7-C antikoerper, LIN-7C antikoerper, MALS-3 antikoerper, MALS3 antikoerper, VELI3 antikoerper, 9130007B12Rik antikoerper, AI303698 antikoerper, AU019331 antikoerper, AW125731 antikoerper, D2Ertd520e antikoerper, Veli3 antikoerper, lin-7 homolog C, crumbs cell polarity complex component antikoerper, lin-7 homolog C L homeolog antikoerper, lin-7 homolog C (C. elegans) antikoerper, LIN7C antikoerper, lin7c.L antikoerper, lin7c antikoerper, Lin7c antikoerper

Hintergrund: Name/Gene ID: LIN7C

Synonyms: LIN7C, LIN-7 protein 3, LIN-7-C, VELI3, Protein lin-7 homolog C, Vertebrate lin-7 homolog 3, Lin-7 homolog C (C. elegans), LIN-7C, MALS-3, MALS3, Mammalian lin-seven protein 3, Veli-3

Gen-ID: 55327

NCBI Accession: NP_060832

UniProt: Q9NUP9

Pathways: Synaptic Membrane